Publication
Garcia C, Delprat A, Ruiz A, Valente VL
G3 (Bethesda, Md.) (Volume: 5; Issue: 12)
2015 Oct 7
G3 (Bethesda, Md.) (Volume: 5; Issue: 12)
2015 Oct 7
Participating groups
Drosophila Evolutionary Genetics
Members
Abstract
Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromosomes based on a handful of genetic and physical markers. This paucity of markers was particularly striking in the metacentric chromosome II, comprised two similarly sized arms, IIL and IIR, traditionally considered homologous to Muller elements C and B, respectively. In this paper we present the cytological mapping of 22 new gene markers to increase the number of markers mapped by in situ hybridization and to test the assignment of scaffolds to the polytene chromosome II arms. For this purpose,...